Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
نویسندگان
چکیده
منابع مشابه
Development of a Multiplex RT-PCR Assay for Detection of the Causal Agents of Citrus Tristeza and Cachexia Diseases with Coamplification of Plant mRNA as an Internal Control
Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...
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Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry. In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). T...
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Hepatitis Delta virus (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV; genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequ...
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Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract infection. At present, both are still diagnosed in the laboratory retrospectively by serology. This is despite many publications which indicate that PCR, which is not retrospective, is extremely good at detecting these organisms. We thought that a single PCR test which could detect both organisms simultaneously in a...
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ژورنال
عنوان ژورنال: Ciencia e investigación agraria
سال: 2014
ISSN: 0718-1620
DOI: 10.4067/s0718-16202014000200012